LOCUS       NC_008119              10778 bp    DNA     circular BCT 08-DEC-2007
DEFINITION  Yersinia pestis Nepal516 plasmid pPCP, complete sequence.
ACCESSION   NC_008119
VERSION     NC_008119.1  GI:108793837
KEYWORDS    .
SOURCE      Yersinia pestis Nepal516
  ORGANISM  Yersinia pestis Nepal516
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Yersinia.
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Chain,P.S., Hu,P., Malfatti,S.A., Radnedge,L., Larimer,F.,
            Vergez,L.M., Worsham,P., Chu,M.C. and Andersen,G.L.
  TITLE     Complete Genome Sequence of Yersinia pestis Strains Antiqua and
            Nepal516: Evidence of Gene Reduction in an Emerging Pathogen
  JOURNAL   J. Bacteriol. 188 (12), 4453-4463 (2006)
   PUBMED   16740952
REFERENCE   2  (bases 1 to 10778)
  AUTHORS   Barry,K., Lucas,S., Lapidus,A., Copeland,A., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Dalin,E., Tice,H., Pitluck,S., Chain,P.,
            Hu,P., Malfatti,S.A., Radnedge,L., Vergez,L.M., Larimer,F.,
            Land,M., Hauser,L., Worsham,P., Chu,M.C., Andersen,G. and
            Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Plasmid pPCP of Yersinia pestis Nepal516
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10778)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2006) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   4  (bases 1 to 10778)
  AUTHORS   Barry,K., Lucas,S., Lapidus,A., Copeland,A., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Dalin,E., Tice,H., Pitluck,S., Chain,P.,
            Hu,P., Malfatti,S.A., Radnedge,L., Vergez,L.M., Larimer,F.,
            Land,M., Hauser,L., Worsham,P., Chu,M.C., Andersen,G. and
            Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2006) US DOE Joint Genome Institute, 2800
            Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CP000307.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 2773192
            Source DNA and bacteria available from Gary Andersen
            (glandersen@lbl.gov)
            Contacts: Gary Andersen (glandersen@lbl.gov)
                      Paul Richardson (microbes@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL
            Finishing done by JGI-LLNL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10778
                     /organism="Yersinia pestis Nepal516"
                     /mol_type="genomic DNA"
                     /strain="Nepal516"
                     /db_xref="taxon:377628"
                     /plasmid="pPCP"
     gene            81..1103
                     /locus_tag="YPN_PCP0001"
                     /db_xref="GeneID:4122532"
     CDS             81..1103
                     /locus_tag="YPN_PCP0001"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="YP_636674.1"
                     /db_xref="InterPro:IPR007101"
                     /db_xref="GI:108793838"
                     /db_xref="GeneID:4122532"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1103..1882
                     /locus_tag="YPN_PCP0002"
                     /db_xref="GeneID:4122533"
     CDS             1103..1882
                     /locus_tag="YPN_PCP0002"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase/IS protein"
                     /protein_id="YP_636675.1"
                     /db_xref="InterPro:IPR001270"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="GI:108793839"
                     /db_xref="GeneID:4122533"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            2919..3113
                     /locus_tag="YPN_PCP0008"
                     /db_xref="GeneID:4122539"
     CDS             2919..3113
                     /locus_tag="YPN_PCP0008"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein"
                     /protein_id="YP_636676.1"
                     /db_xref="InterPro:IPR000769"
                     /db_xref="GI:108793845"
                     /db_xref="GeneID:4122539"
                     /translation="MNKQQQTALNMARFIRSQSLILLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQARFEAESETGT"
     gene            4086..4280
                     /locus_tag="YPN_PCP0009"
                     /db_xref="GeneID:4122540"
     CDS             4086..4280
                     /locus_tag="YPN_PCP0009"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein"
                     /protein_id="YP_636677.1"
                     /db_xref="InterPro:IPR000769"
                     /db_xref="GI:108793846"
                     /db_xref="GeneID:4122540"
                     /translation="MNKQQQTALNMARFIRSQSLILLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQARFEAESETGT"
     gene            complement(5976..7049)
                     /locus_tag="YPN_PCP0003"
                     /db_xref="GeneID:4122534"
     CDS             complement(5976..7049)
                     /locus_tag="YPN_PCP0003"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin"
                     /protein_id="YP_636678.1"
                     /db_xref="GI:108793840"
                     /db_xref="GeneID:4122534"
                     /translation="MSDTMVVNGSGGVPAFLFSGSTLSSYRPNFEANSITIALPHYVD
                     LPGRSNFKLMYIMGFPIDTEMEKDSEYSNKIRQESKISKTEGTVSYEQKITVETGQEK
                     DGVKVYRVMVLEGTIAESIEHLDKKENEDILNNNRNRIVLADNTVINFDNISQLKEFL
                     RRSVNIVDHDIFSSNGFEGFNPTSHFPSNPSSDYFNSTGVTFGSGVDLGQRSKQDLLN
                     DGVPQYIADRLDGYYMLRGKEAYDKVRTAPLTLSDNEAHLLSNIYIDKFSHKIEGLFN
                     DANIGLRFSDLPLRTRTALVSIGYQKGFKLSRTAPTVWNKVIAKDWNGLVNAFNNIVD
                     GMSDRRKREGALVQKDIDSGLLK"
     gene            7279..7584
                     /locus_tag="YPN_PCP0004"
                     /db_xref="GeneID:4122535"
     CDS             7279..7584
                     /locus_tag="YPN_PCP0004"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_636679.1"
                     /db_xref="GI:108793841"
                     /db_xref="GeneID:4122535"
                     /translation="MSKTKSGRHRLSKTDKRLLAALVVAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKYLSGKKPEGVEPREGQEREDLP"
     gene            7827..8765
                     /locus_tag="YPN_PCP0005"
                     /db_xref="GeneID:4122536"
     CDS             7827..8765
                     /locus_tag="YPN_PCP0005"
                     /EC_number="3.4.23.48"
                     /note="outer membrane protease; involved in virulence in
                     many organisms; OmpT; IcsP; SopA; Pla; PgtE; omptin; in
                     Escherichia coli OmpT can degrade antimicrobial peptides;
                     in Yersinia Pla activates plasminogen during infection; in
                     Shigella flexneria SopA cleaves t"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protease"
                     /protein_id="YP_636680.1"
                     /db_xref="InterPro:IPR000036"
                     /db_xref="InterPro:IPR010916"
                     /db_xref="GI:108793842"
                     /db_xref="GeneID:4122536"
                     /translation="MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLS
                     GKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNM
                     DDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFS
                     WTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSD
                     WVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGG
                     TQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF"
     sig_peptide     7827..7889
                     /locus_tag="YPN_PCP0005"
                     /product="outer membrane protease"
                     /note="Signal predicted by SignalP 2.0 HMM (Signal peptide
                     probability 1.000) with cleavage site probability 0.997 at
                     residue 21"
     gene            complement(8952..9251)
                     /locus_tag="YPN_PCP0006"
                     /db_xref="GeneID:4122537"
     CDS             complement(8952..9251)
                     /locus_tag="YPN_PCP0006"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="YP_636681.1"
                     /db_xref="InterPro:IPR001387"
                     /db_xref="GI:108793843"
                     /db_xref="GeneID:4122537"
                     /translation="MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQK
                     QVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGRRVAFHV"
     gene            complement(9251..9598)
                     /locus_tag="YPN_PCP0007"
                     /db_xref="GeneID:4122538"
     CDS             complement(9251..9598)
                     /locus_tag="YPN_PCP0007"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_636682.1"
                     /db_xref="GI:108793844"
                     /db_xref="GeneID:4122538"
                     /translation="MWMVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYA
                     DTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVLCAGDKSNDKRFYEKLVRIAE
                     DEFTAHLNTLESK"
ORIGIN      
        1 gaacggtgca atagtgatcc acacccaacg cctgaaatca gatccagggg gtaatctgct
       61 ctcctgattc aggagagttt atggtcactt ttgagacagt tatggaaatt aaaatcctgc
      121 acaagcaggg aatgagtagc cgggcgattg ccagagaact ggggatctcc cgcaataccg
      181 ttaaacgtta tttgcaggca aaatctgagc cgccaaaata tacgccgcga cctgctgttg
      241 cttcactcct ggatgaatac cgggattata ttcgtcaacg catcgccgat gctcatcctt
      301 acaaaatccc ggcaacggta atcgctcgcg agatcagaga ccagggatat cgtggcggaa
      361 tgaccattct cagggcattc attcgttctc tctcggttcc tcaggagcag gagcctgccg
      421 ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact atgcgtaatg
      481 gtcgctcacc gcttcacgtg ttcgttgctg ttctcggata cagccgaatg ctgtacatcg
      541 aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat gcgttccgct
      601 tctttggtgg tgtgccgcgc gaagtgttgt atgacaatat gaaaactgtg gttctgcaac
      661 gtgacgcata tcagaccggt cagcaccggt tccatccttc gctgtggcag ttcggcaagg
      721 agatgggctt ctctccccga ctgtgtcgcc ccttcagggc acagactaaa ggtaaggtgg
      781 aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact cgcctgcgcc
      841 cgatggggat cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc tggctgcacg
      901 atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgcgat cgctggctcg
      961 aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac gtgcatcttg
     1021 atgaaaatct ggtgaacttc gacaaacacc ccctgcatca tccactctcc atctacgact
     1081 cattctgcag aggagtggcg tgatgatgga actgcaacat caacgactga tggcgctcgc
     1141 cgggcagttg caactggaaa gccttataag cgcagcgcct gcgctgtcac aacaggcagt
     1201 agaccaggaa tggagttata tggacttcct ggagcatctg cttcatgaag aaaaactggc
     1261 acgtcatcaa cgtaaacagg cgatgtatac ccgaatggca gccttcccgg cggtgaaaac
     1321 gttcgaagag tatgacttca cattcgccac cggagcaccg cagaagcaac tccagtcgtt
     1381 acgctcactc agcttcatag aacgtaatga aaatatcgta ttactggggc catcaggtgt
     1441 ggggaaaacc catctggcaa tagcgatggg ctatgaagca gtccgtgcag gtatcaaagt
     1501 tcgcttcaca acagcagcag atctgttact tcagttatct acggcacaac gtcagggccg
     1561 ttataaaacg acgcttcagc gtggagtaat ggccccccgc ctgctcatca ttgatgaaat
     1621 aggctatctg ccgttcagtc aggaagaagc aaagctgttc ttccaggtca tcgctaaacg
     1681 ttacgaaaag agcgcaatga tcctgacatc caatctgccg ttcgggcagt gggatcaaac
     1741 gttcgccggt gatgcagcac tgacctcagc gatgctggac cgtatcttac accactcaca
     1801 tgtcgttcaa atcaaaggag aaagctatcg actcagacag aaacgaaagg ccggggttat
     1861 agcagaagct aatcctgagt aaaacggtgg atcaatattg ggccgttggt ggagatataa
     1921 gtggatcact tttcatccgt cgttgacacc ctgatgaatt cacgtgttca cgcctgaata
     1981 acaagaatgc cggagatacg cagtcatatt ttttacacaa ttctctaatc ccgacaaggt
     2041 cgtaggtcgt tataggaaaa ttcttagcac cattccggaa caatcagaac agcaggccat
     2101 gaacgactga caacattacg aatataaaaa acgcacccgg gccagacatt ccccctactg
     2161 attaaaccag ccggacttgt ccacggaacg gtctttttaa accgacacac agtctgagta
     2221 cagatacatg tcacgatgat gcaggattag cggaagagtg tgagcacgtt tccgggaact
     2281 gtggtgaacc atagctcaat attcgagtga gggcataccg gaaacgcgct cagattcgtt
     2341 gtaacgcgat tttccgtacc gggcaatttt ttcagttgtt ttttcgtttc atgtcgtcag
     2401 aaacgttctg agcgcgtttc cggcatctga tgctacgcaa accatcccca tggtcagttg
     2461 acagccggaa acacgcgggt gtcgttttag cgtatcgacg ggacggcgtc gagaagcaca
     2521 aaaaacagat gttgtactca gtcagttgtt ttacagacag cactgcggca gattgaaaaa
     2581 gtaccgtact ttcaggaatg tccagaaacc atgtgtcaga cttcgttctc ccccttccgg
     2641 gtgaattttt ttgtcatccg ttcaggaatc tctttataac gattactcca tttcaggatt
     2701 ttttatgtgg cgtttactac aggcaggata ttcaaaggca aaaaaatccc ccggaacagg
     2761 cggaacccgg acagggggag aacgaatcgc taaataattt tcgtagttgt atttcccatc
     2821 gttgctactg caacgggatg aatttgccgc agtttatcct gtaaaacaat cctgatttac
     2881 tcacactcca catatcactg acggagcaca acggaatagt gaacaaacaa caacaaactg
     2941 cgctgaatat ggcgcgattt atcagaagcc agagcctgat actgcttgaa aaactggatg
     3001 ctctggatgc cgacgagcag gcggccatgt gtgaacgact gcacgaactc gcggaagaac
     3061 tccagaacag catccaggct cgctttgaag ccgaaagtga aacaggaaca taacgacctg
     3121 atgaattcac gtgttcacgc ctgaataaca agaatgccgg agatacgcag tcatattttt
     3181 tacacaattc tctaatcccg acaaggtcgt aggtcgttat aggaaaattc ttagcaccat
     3241 tccggaacaa tcagaacagc aggccatgaa cgactgacaa cattacgaat ataaaaaacg
     3301 cacccgggcc agacattccc cctactgatt aaaccagccg gacttgtcca cggaacggtc
     3361 tttttaaacc gacacacagt ctgagtacag atacatgtca cgatgatgca ggattagcgg
     3421 aagagtgtga gcacgtttcc gggaactgtg gtgaaccata gctcaatatt cgagtgaggg
     3481 cataccggaa acgcgctcag attcgttgta acgcgatttt ccgtaccggg caattttttc
     3541 agttgttttt tcgtttcatg tcgtcagaaa cgttctgagc gcgtttccgg catctgatgc
     3601 tacgcaaacc atccccatgg tcagttgaca gccggaaaca cgcgggtgtc gttttagcgt
     3661 atcgacggga cggcgtcgag aagcacaaaa aacagatgtt gtactcagtc agttgtttta
     3721 cagacagcac tgcggcagat tgaaaaagta ccgtactttc aggaatgtcc agaaaccatg
     3781 tgtcagactt cgttctcccc cttccgggtg aatttttttg tcatccgttc aggaatctct
     3841 ttataacgat tactccattt caggattttt tatgtggcgt ttactacagg caggatattc
     3901 aaaggcaaaa aaatcccccg gaacaggcgg aacccggaca gggggagaac gaatcgctaa
     3961 ataattttcg tagttgtatt tcccatcgtt gctactgcaa cgggatgaat ttgccgcagt
     4021 ttatcctgta aaacaatcct gatttactca cactccacat atcactgacg gagcacaacg
     4081 gaatagtgaa caaacaacaa caaactgcgc tgaatatggc gcgatttatc agaagccaga
     4141 gcctgatact gcttgaaaaa ctggatgctc tggatgccga cgagcaggcg gccatgtgtg
     4201 aacgactgca cgaactcgcg gaagaactcc agaacagcat ccaggctcgc tttgaagccg
     4261 aaagtgaaac aggaacataa cgaagctccc ggagacggtc acagcttgtc tgtgaacgga
     4321 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt tttagcgggt gtcggggcgc
     4381 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttagtca tgcggcatca
     4441 gtgcggattg tatgaaaagt gcaccatgta cggtgtgaaa tgccgcacag atgcgtaagg
     4501 agaacatgca gatgccgatg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4561 gttcggctgc ggcgagcggt gtctgctcac tcaaaagcgg tgatactgtt atccacacaa
     4621 tcaggggata acgccggaaa gaacatgtga gcaaaaaacg aagaccccag aaaaggccgc
     4681 gccggaggcg ctttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4741 caagtcagag gtggcgaaac ccgacaggac ttaaagatac caggcgtttc cccccggaag
     4801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctct ccgcctttct
     4861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt tggtatctca gttcggtgta
     4921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accactgcgc
     4981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttta cgccactggc
     5041 agcagccatt ggtaactgaa aagtggattt agatacgcag aactcttgaa gttgaagcct
     5101 tatcgcggct acactgaaag gacagcattt ggtatctgtg ctccacttaa gccagctacc
     5161 acaggttaga aagcctgaga aacttctaac cttcgaaaga acccacgcct gagaacgtgg
     5221 gttttttcgt ttacaggcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5281 tgatcttttc tactgaattg cgctcccgat cagttcagca gaagattatg atggggttct
     5341 atgggtattg ctgcggtaac acccatgtta cttgaggttg tatgtagtct gtgtagaatt
     5401 atacacataa ggcttaaact gctctttttt ttcaatatgc aattggaagt tcattgacta
     5461 cataaataga ttattccaaa taatttattt atgtaagaac aggatgggag ggggaatgat
     5521 ctcaaagtta ttttgcttgg ctctcatatt tttatcatca agtggccttg cagaaaaaaa
     5581 cacatataca gcaaaagaca tcttgcaaaa cctagaatta aatacctttg gcaattcatt
     5641 gtctcatggc atctatggga aacagacaac cttcaagcaa accgagttta caaatattaa
     5701 aagcaacacc aaaaaacaca ttgcacttat caataaagac aactcatgga tgatatcatt
     5761 aaaaatacta ggaattaaga gagatgagta tactgtctgt tttgaagatt tctctctaat
     5821 aagaccgcca acatatgtag ccatacatcc tctacttata aaaaaagtaa aatctggaaa
     5881 ctttatagta gtgaaagaaa taaagaaatc tatccctggt tgcactgtat attatcatta
     5941 atagcaagcc cctcattatt atgaggggct catggttatt ttaacaatcc actatcgata
     6001 tctttttgca ccagagcgcc ctctcgttta cgtctgtcag acattccatc aacaatatta
     6061 ttaaaagcat ttacaaggcc attccagtct tttgcgataa ctttattcca tactgtggga
     6121 gcagttctgg ataacttaaa ccctttttga tatccaatag acaccagtgc tgtacgggtt
     6181 ctcaacggta aatcgctgaa ccgaagaccg atattagcgt cattgaaaag accttcaatc
     6241 ttatgtgaga atttatcaat ataaatatta gataagagat gagcttcatt atcagaaagc
     6301 gtcagaggtg ctgttctcac tttatcataa gcctccttcc ctcgaagcat ataataccca
     6361 tcaagtctat ctgcaatata ctgagggaca ccgtcattca ataaatcctg tttgcttcgc
     6421 tgaccaaggt caaccccgga accgaatgta acaccggtac tgttaaaata atcgctacta
     6481 ggattagacg gaaaatgact tgtcggatta aacccttcaa aaccattact ggagaaaata
     6541 tcgtggtcaa caatatttac cgaacgacgt aaaaattcct tcagttgact aatattgtca
     6601 aagttaatga cagtgttgtc cgctaggacg atgcgatttc ggttattatt cagaatgtct
     6661 tcgttctctt tcttatcgag atgttcaata gattcggcaa tcgttccctc aagaaccatg
     6721 acacggtaga ctttcacacc gtctttttcc tgacctgttt caacagttat tttctgttcg
     6781 taagacacgg tcccttcagt ttttgaaatt ttactttcct ggcggatctt atttgaatat
     6841 tcactgtctt tctccatctc cgtatcaatc ggaaacccca taatgtacat cagtttaaaa
     6901 ttactccggc caggcagatc cacataatgt ggtaatgcaa ttgtaatcga attagcttca
     6961 aaatttggtc tgtaactgct taatgtactt ccggaaaaga gaaaagccgg aacaccacct
     7021 gaaccattca ctaccattgt atctgacata aaaattcctc tttaacacat aaaaaaaaca
     7081 ataagttaaa aaaaaatact gtacataaaa ccactgtttt tatgtacagt aataaaatta
     7141 cgccgcttta ttttctctgt caataatatg aaatttcatt tttgtgatct gaatcactct
     7201 tataaaaatc aggaagggaa gattcgcagc agaaaaacag caccgggtaa catcagaaaa
     7261 aaacagaaag gagataacgt gagcaaaaca aaatctggtc gccaccgact gagcaaaaca
     7321 gacaaacgcc tgctggctgc acttgtcgtt gccggatacg aagaacggac agcccgtgac
     7381 ctcatccaga aacacgttta cacactgaca caggccgacc tgcgccatct ggtcagtgaa
     7441 atcagtaacg gtgtgggaca gtcacaggcc tacgatgcga tttaccaggc gagacgcatt
     7501 cgtctcgccc gtaaatacct gagcggaaaa aaaccggaag gggtggaacc ccgggaaggg
     7561 caggaacggg aagatttacc ataactcccg ttatcagtac catcggctca acgctcgttg
     7621 tcggatctga aaaattcgct caaaagatca tatttccctg gatattttcc accgtttctt
     7681 atgtgagcaa agtcacataa ttctgtcaga cgacgagaaa acggatatcg attattgttt
     7741 aatattttta cattattaaa aatgaaatta gataatcaga tacaaataat atgttttcgt
     7801 tcatgcagag agattaaggg tgtctaatga agaaaagttc tattgtggca accattataa
     7861 ctattctgtc cgggagtgct aatgcagcat catctcagtt aataccaaat atatcccctg
     7921 acagctttac agttgcagcc tccaccggga tgctgagtgg aaagtctcat gaaatgcttt
     7981 atgacgcaga aacaggaaga aagatcagcc agttagactg gaagatcaaa aatgtcgcta
     8041 tcctgaaagg tgatatatcc tgggatccat actcatttct gaccctgaat gccagggggt
     8101 ggacgtctct ggcttccggg tcaggtaata tggatgacta cgactggatg aatgaaaatc
     8161 aatctgagtg gacagatcac tcatctcatc ctgctacaaa tgttaatcat gccaatgaat
     8221 atgacctcaa tgtgaaaggc tggttactcc aggatgagaa ttataaagca ggtataacag
     8281 caggatatca ggaaacacgt ttcagttgga cagctacagg tggttcatat agttataata
     8341 atggagctta taccggaaac ttcccgaaag gagtgcgggt aataggttat aaccagcgct
     8401 tttctatgcc atatattgga cttgcaggcc agtatcgcat taatgatttt gagttaaatg
     8461 cattatttaa attcagcgac tgggttcggg cacatgataa tgatgagcac tatatgagag
     8521 atcttacttt ccgtgagaag acatccggct cacgttatta tggtaccgta attaacgctg
     8581 gatattatgt cacacctaat gccaaagtct ttgcggaatt tacatacagt aaatatgatg
     8641 agggcaaagg aggtactcag accattgata agaatagtgg agattctgtc tctattggcg
     8701 gagatgctgc cggtatttcc aataaaaatt atactgtgac ggcgggtctg caatatcgct
     8761 tctgaaaaat acagatcata tctctctttt catcctcccc tagcggggag gatgtctgtg
     8821 gaaaggaggt tggtgtttga ccaaccttca gatgtgtgaa aaatcacctt tttcaccata
     8881 atgacggggc gctcattctg ttgttttgcc ttgacattct ccacgtcttt cagggcatgg
     8941 agaaggtcaa attagacatg gaacgctact ctccttcctg taggaagctc aacatccaag
     9001 cttaatttgc ctcccattgc ttcaacgtaa cgctttaacg tcgccagctt taaatcattt
     9061 ccgcgctgct ccagctttgt tactgctggc tggcttatac ccatcgcctc agcaacttgt
     9121 ttttgtgata actggagttc ttcacgcatc atctgcaagc cgacctcaag aatcatctca
     9181 tctgccattt ctttaattcg tgtctggctt tcaggtgaac gactggcaat cacctcatct
     9241 aatgttctca ttacttgctc tccagtgtgt tcagatgtgc tgtaaattca tcctcagcta
     9301 tacgcaccag tttttcataa aaccgcttat cattactttt atctcctgca caaagaacga
     9361 tagcccgacg aatcggatcg aacgcataaa aggctcttat cggacggcca gaaaactgaa
     9421 cgcgaagctc tttcatattt ttgtaccgag aacctttcac ggtatcggca tatggcctgg
     9481 gtaactcagg tccgtaaacc tgtagctttt tcaaatcagc caaaaccttt tcctgaagag
     9541 cgtcttcttg ctcatttagc cagtcgtcaa atcgctggct aaaaagtacc atccacatgc
     9601 tcaaccctat aacctgtagc ttaccccact aacaatataa cctacgagtt atattttcaa
     9661 gaaaagctgg ctatttaaca taacggcaat ttgtacgcac cactgaaatg cgttcagcgc
     9721 gatcacggca acagacaggc aaaaatagca acaaacctcc cgaaaaaccg ccgcgatcgc
     9781 gcctgataaa ttttaacctt atgcatatct atgcagccag gcgaatcacg aacgaattgc
     9841 ctgcctgatg taactgaaac gggtgttttt tcctgatttg gtgggcgtgg aagacggaac
     9901 atgaacggga aaacagaatt catgccagat gagcgcgatc tggcaattaa ggcaaaacac
     9961 agcaacaaag acacgccaga atcgcgcccg gatatgtttt aacgcgattt tcagactcag
    10021 acaaattcag cagaatgcta ctccattcac cgggctgatg gtgaatacat gcgtatccag
    10081 gatgagtaca tttctggctc tgccacagct ctgtctgttg gcagctttcg cctgtccgga
    10141 aacctgctta aaacgctccc gaaaggcctc tgaaccagaa agcaacaaaa cacaggccat
    10201 taagtaaatc gcgttaaaac acgtctgatg gattgctgca aaaaaaagtc cctaatggag
    10261 cagggactgt taaacccagt gaatagcgtc taaattaaag taagaatacg accaggtact
    10321 cttcagaaaa gagattaatc caccgcacag aataatcaac agtaaaaaca aacaaccctg
    10381 attttttatt tttctttttt tcgataaaaa caaaattaaa gaaataatta atcagaacat
    10441 tccttaactt cagggcattg cctgtgttcc attttgtgat tagtctgaaa cttccgaagg
    10501 tggataacac ccggtatttt tttgctcaca taaagcccct ccttcaggca gaggggcttt
    10561 ttctttgcca ccacataaaa aaggccctca caggaggtgt tctgtgaggg cgtatgataa
    10621 ggactgaatc gatggttaat atgtctagtc ctgacttttg catctccgaa tataaaaccc
    10681 tgtttaacgg catgcaaaac caaaaaataa aaatgtgaca tcgcaatgcc agataatatt
    10741 gacgcatgag ggaatgcgta ccccgacccc tgtgtaac
//
